Abstract:

Background and Aim: The presence of anti-biotic resistant Klebsiella pneumoniae in patients’ causes treatment failure with a wide range of common antibiotics, especially in ICU. This study aims to determine the molecular identification of anti-biotic resistant genes in Klebsiella pneumoniae sampled from the respiratory system of the patients admitted to ICU.

Materials and Methods: In this descriptive-sectional study, a total of 50 purposive samples of respiratory secretions of the ICU patients in Kerman hospitals were studied in a 5 months period. The anti-biotic susceptibility to 8 antibiotics ceftazidime, cefotaxime, ceftriaxone, imipenem, ciprofloxacin, amikacin, aztreonam, and cephalothin was determined. PCR test to identify SHV and TEM genes performed and the molecular identification of the antibiotic resistance genes of these isolates were determined.

Results: Imipenem has the highest anti-biotic susceptibility (94%), and cephalotin has the highest resistance (64%) among the isolates. 56% of the isolates were positive ESBL phonotype generating. In 28 cases of ESBL generating isolates, 25% had only the bla_SHV gene, 64.3% had both the bla_TEM and bla_SHV genes, and 10.7 % had neither of these genes also none of the isolates had only the bla_TEM gene.

&zwjConclusion: Klebsiella pneumoniae of the contained patients admitted, rapid and accurate molecular techniques in the identification of genetic resistance is necessary. The prevalence of resistant strains of bacteria has been assessed and appropriate measures for the treatment and control of bacterial resistance to be implemented.