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Detection luxS, qseC and rsbA genes’ bands in Proteus mirabilis and Escherichia coli isolated from urinary tract infections
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Sara Ahmadi Badi *   , Jamileh Nowroozi , Abbas Akhavan Sepahi |
| Faculty of Basic Sciences, Islamic Azad University, North Tehran Branch, Tehran, Iran; Phone: +98 9124044708 , sarahmadi@gmail.com |
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Abstract: (7228 Views) |
| Background and Aim: Considering the importance of quorum sensing, its prominent role in virulence of bacteria and considering urinary infection commonality and the known complications of these diseases and properties of luxS, qseC, and rsbA gene’s bands in communicating among bacterial species, the present paper was conducted on the laboratory urinary samples in 1392. Materials and Methods: The present study was done by cross sectional method on 100 urinary samples collected from various laboratory. Bacterial culture and Proteus mirabilis and Escherichia coli were detected by means of specific methods diagnosed urinary infection. The DNA of these bacteria was extracted. The existence of luxS, qseC, and rsbA gene’s bands were studied by the PCR method and presented by descriptive statistics. Results: In 100 urine sample studied, 45 Escherichia coli (45%) and 10 Proteus mirabilis (10%) were isolated. In 60% studied E. coli contained luxS genes’ band and 90% contained qseC genes’ band. Also, 70% studied P. mirabilis, contained luxS and rsbA genes’ band. Conclusion: It seems that the existence of the above-mentioned gene bands (specially luxS gene’s band) are common in these bacteria, which are responsible for urinary infection. The study of the effect of these genes expression, suppress on virulence is suggested. |
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| Keywords: Quorum sensing, Urinary tract infections, luxS gene, qseC gene, rsbA gene, Proteus mirabilis, Escherichia coli |
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Full-Text [PDF 438 kb]
(1846 Downloads)
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Type of Study: Original |
Subject:
Medicine
Received: 2017 | Accepted: 2017 | Published: 2017
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