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Synthesis of silver nanoparticle from silver nitrate using culture extract of Rhodotorula sp. strain GM5
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Morahem Ashengroph *   |
| Assistant Professor of Microbiology, Department of Biology and Biotechnology, Faculty of Sciences, University of Kurdistan, Sanandaj, Iran , m.ashengroph@uok.ac.ir |
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Abstract: (9376 Views) |
| Background and Aim: Nanotechnology is a manipulating matter on a near-atomic scale to provide new approach for combating infectious diseases. Silver nanoparticles are increasingly being used in medicine, pharmaceutical, and health sciences for its antimicrobial unique properties. Today, biological synthesis of nanoparticles is considered as an environmentally friendly and inexpensive method. Although the toxic effects of silver ions for most microorganisms have been reported, but several species of the microorganisms including the native screened yeast strain used in this project can overcome the toxicity of the metal ion by its reduction to the elemental form, to form nano-silver. The present study evaluates the potential use of novel yeast strains as a novel biocatalyst to reduce silver nitrate to silver nanoparticles in aqueous form. Materials and Methods: Eleven different strains of yeasts were isolated from collecting soil samples from near gold mines of Ghorveh in Iran's Kurdistan Province using conventional enrichment process and characterized initially based on macroscopic and microscopic characteristics and preliminary biochemical tests. The supernatants of the isolated yeasts were incubated with AgNO3 solution (2.5 mM) in a shaker incubator for 48h the strain that were able to synthesis of silver nanoparticle was identified. The silver nanoparticles formation was investigated through UV-visible spectroscopic techniques, XRD, SEM microscopic observations and size distribution of nanoparticles were determined by particle size analyzer Results: Among the screened yeast strains, the strain GM5, showed the capability of promoting the extracellular formation of Ag nanoparticles. The strain was selected and identified as Rhodotorula (GenBank accession number KF543865) based on morphological and biochemical characteristics and its molecular phylogenetic analysis. Results obtained by visual observations, spectral data achieved from UV–vis, XRD spectrum, particle size analyzer and SEM micrographs confirmed the extracellular formation of spherical silver nanoparticles with spherical morphology and narrow size distribution with average particle size of 40 nm by using the culture supernatant of Rhodotorula sp. strain GM5. Conclusion: Extracellular synthesis of silver nanoparticles, without the need for complicated extraction steps, can be taken by using the culture supernatants of Rhodotorula sp. strain GM5. This biologic method has the potential to replace chemical and physical methods used for synthesis of silver nanoparticles and can be efficiently used as an eco-friendly biocatalyst for the preparative synthesis of Ag nanoparticles. The present study is the first report on the biological synthesis of Ag nanoparticles using genus of Rhodotorula. |
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| Keywords: Silver nanoparticle, Biological reduction, Rhodotorula sp. strain GM5 |
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Full-Text [PDF 633 kb]
(3040 Downloads)
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Type of Study: Original |
Subject:
Medicine
Received: 2017 | Accepted: 2017 | Published: 2017
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