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Is there a possible way to accurately determine biochemical parameters in lipemic specimens?
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Houshang Amirrasouli *   , Naser Valai |
| Associate Professor, Department of laboratory medicine, School of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran , houshangan@mail.sbmu.com |
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Abstract: (9655 Views) |
| Background and Aim: Determination of certain biochemical parameters in hyperlipemic speciemens causes problems, which is due to interference of lipids. Lipid extraction using ultracentrifuge is expensive and is not always available. Another method of lipid extraction is by using ethylacetate thus, we compared certain biochemical parameter values in lipemic specimens and in the same samples after lipid extraction with ethylacetate. Materials and methods: In this experimental study, 45 normal serum samples were selected. To obtain lipemic specimens, lipid was added to these samples. Each serum sample was then divided into two aliquots in one sample lipid was extracted using ethylacetate while in the other one ultracentrifugation was used. Later, 13 biochemical parameters were determined in these lipid free samples and their results were compared using paired samples t-Test. Results: While the biochemical parameters of the samples were normal at the beginning, they were measured as abnormal after lipid addition. There were no significant difference between values obtained by ethylacetate extraction method and standard ultracentrifugation method (P<0.6). Although urea value was lower & ALT was higher in ethylacetate extraction method, their values were closer to the original specimen compared to ultracentrifuge. Conclusion: It seems that extraction of lipids from lipemic speciemens using ethylacetate is a feasible method however, further studies regarding this issue is recommended. |
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| Keywords: Hyperlipidemias, Lipemic, Biochemical Parameters, Ethyl Acetate |
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Full-Text [PDF 88 kb]
(4743 Downloads)
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Type of Study: Original |
Subject:
Medicine
Received: 2017 | Accepted: 2017 | Published: 2017
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