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NOD2 Gene Exonic Variations in Iranian Patients with Crohn's Disease
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Rahim Aghazadeh3, Faramarz Derakhshan1, Mohammad Reza Zali   , Nosratollah Naderi , Alma Farnood * , Manigeh Habibi , Hedieh Balaii , Homayoun Zojaji , Mohsen Chiani , Ali Tahami , Farzad Firouzi , Masoumeh Soltani |
| , a_farnood@yahoo.com |
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Abstract: (9027 Views) |
| Background and Aim: The NOD2 gene is known to have a strong association with Crohn’s disease, but different trends were reported in occurrence of NOD2 variants in distinct ethnicities. The aim of this study was to assess all exonic sequences of the NOD2 gene in Iranian Crohn's patients and healthy controls to identify any existing variation and evaluate their association with Crohn's disease. Materials and Methods: In the present study 90 non-related Crohn's patients and 120 sex- and age- matched healthy controls were included. They were referred to a tertiary center in a three-year period (2006-2009). The exonic regions of the NOD2 gene were amplified by polymerase chain reaction and evaluated by direct sequencing. Results: Totally, 21 sequence variations were identified among all exonic regions of the NOD2 gene, among which 8 had an allele frequency more than 5%. Eight new mutations (1 in exon 2 and 7 in exon 4) were observed. The three main variants (R702W, G908R, and 1007fs) showed allele frequencies of 13.3%, 2.2%, and 1.7%, respectively. Three new variations (P371T, A794P, and Q908H) and R702W mutation were significantly more frequent in Crohn's patients compared to controls. Conclusion: Eight novel mutations were identified in the NOD2 exons, but the patho-physiological importance of these variants remains unclear. Iranian patients with their different genetic reservoir may demonstrate some novel characteristics for disease susceptibility. |
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| Keywords: NOD2, Crohn’s Disease, Mutation. |
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Full-Text [PDF 209 kb]
(1777 Downloads)
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Type of Study: Original |
Subject:
Medicine
Received: 2017 | Accepted: 2017 | Published: 2017
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