Background and Aim: Recurrent miscarriage, which is defined as 3 or more consecutive pregnancy losses before 20 weeks of gestation, affects 1-5% of woman in the reproductive age group. Endothelial nitric oxide synthase (eNOS) is an enzyme that influences placental human chorionic gonadotropin production during gestation. Previous studies have indicated an association between eNOS activity, implantation, and maintenance of pregnancy, but proposed associations between polymorphisms of the eNOS gene and recurrent miscarriage (RM) are controversial. In this study was examined the association between rs1177443, rs1799983 and VNTR in intron 4 polymorphisms of eNOS gene and abortion in Iranian patients. Materials and Methods: We performed a study of 80 women with two or more pregnancy losses who did not have any other known risk factors as a case group and the control group who were 80 women with at least two healthy children and no RPL in their reproductive history. DNA was extracted from the peripheral blood following written consent. The presence or absence of the mutation for these factors was assessed by PCR and RFLP analysis, using Mnl1 and BanII restriction enzyme, respectively. The data were statistically analyzed using chi square test and SPSS 18. Results: The results showed that no significant associated between rs1177443 (P=0.16), rs1799983 (P=0.15) and VNTR in intron 4 (P=0.48) polymorphisms of eNOS gene and recurrent miscarriage in case and control group. Conclusion: In conclusion, there is no significant difference between mentioned polymorphisms of eNOS gene and recurrent miscarriage.