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Volume 18, Issue 6 (January & February 2014 2014) |
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The study of attachment factor and biofilm formation of uropathogenic Escherichia coli isolated from patient with urinary tract infection of Semnan Province
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Nazila Arbab Soleimani   , Zahra Amini * , Elahe Tajbakhsh |
| Department of Microbiology, Damghan Branch, Islamic Azad University, Damghan, Iran , Zahra_amini_63@yahoo.com |
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Abstract: (8418 Views) |
| Background and Aim: Urinary tract infection (UTI) is the most common nosocomial infection, which is caused by colonization of uropathogenic Escherichia coli to host mucosal epithelium and damage host tissue. Materials and Methods: In this study 100 samples were collected from patients who suspected urinary tract infection. Antibiotic resistance of uropathogenic Escherichia coli was done by Kirby-Bauer method. Biofilm formation of this bacterium was performed by micro-titer plate method. To study the attachment of bacteria, cell surface hydrophobicity of uropathogenic Escherichia coli was done by hydrocarbon octane method. Results: Among 100 examined samples using conventional biochemical methods, 70 samples were identified as uropathogenic Escherichia coli. Results showed that, this bacteria with 23% hydrophobicity was able to form biofilm. Among these, 60% showed strong potential to form biofilm and just 4% did not have such potential. |
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| Keywords: Urinary tract infections, Uropathogenic Escherichia coli, Biofilm formation |
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Full-Text [PDF 302 kb]
(4035 Downloads)
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Type of Study: Original |
Subject:
Medicine
Received: 2017 | Accepted: 2017 | Published: 2017
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Nazila Arbab Soleimani, Zahra Amini, Elahe Tajbakhsh. The study of attachment factor and biofilm formation of uropathogenic Escherichia coli isolated from patient with urinary tract infection of Semnan Province. pajoohande 2014; 18 (6) :332-336 URL: http://pajoohande.sbmu.ac.ir/article-1-1648-en.html
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