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Comparative study of aquaporin-1 protein expression in developing brain of Wistar and H-Tx rats
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Mohammad Nabiuni *   , Javad Rasouli , Azar Sheikholeslami |
| Azar Sheikholeslami , nabiuni@tmu.ac.ir |
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Abstract: (9729 Views) |
| Background and Aim: In congenital hydrocephalus, there is excessive cerebrospinal fluid (CSF) accumulation in the brain that negatively impacts water homeostasis. Aquaporins are a family of transmembrane water channel proteins. They play a significant role in the developing brain and possibly have a role in congenital hydrocephalus. Materials and Methods: We studied the regulation of aquaporin-1 (AQP-1), in the hydrocephalus-Texas (H-Tx) rat model by Immunohistochemistry, western blot analysis, and ELISA assays. Hydrocephalus develops in these animals due to obstruction of the cerebral aqueduct on embryonic day 18, and the condition is fatal by 4-5 weeks of age. AQP-1 protein expression was evaluated in unaffected H-Tx animals, affected hydrocephalic (HC) animal and Wistar control animals at embryonic ages of E17 and E19, and post natal ages of P3 and P10. Results: Our findings indicate that AQP-1 is down-regulated in the congenital H-Tx and affected (HC) animals compared to Wistar control animals. The decrease occurs at day E17, one day before aqueduct stenosis in this animal model. Postnatally, the decrease in AQP-1 is more evident at age P10, suggesting this might be a longstanding phenomenon. Conclusion: AQP-1 may play an important role in the production or absorption of CSF in the early gestation period of congenital hydrocephalus. The molecular aspects of early development in this rodent model may involve an abnormality of aquaporin regulation. The mechanism of AQP-1’s direct involvement in the CSF pathway or its indirect effect on cortical development remains to be understood. |
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| Keywords: Aquaporin-1, Hydrocephalus, H-Tx rat, Cerebrospinal Fluid |
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Full-Text [PDF 1167 kb]
(2756 Downloads)
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Type of Study: Original |
Subject:
Medicine
Received: 2017 | Accepted: 2017 | Published: 2017
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