Abstract:

Background and Aim: Buprenorphine is opium poppy derivative. From curative qualities, this drug is used for the swoon, relief of the acute pain, and in addiction treatment centers. Because of possibility appearance of harmful problems that are created following to using of amphetamine drugs, in this survey the histopathological effects administration of buprenorphine, on testis of male rats were evaluated.

Materials and Methods: In this experimental study, 30 male Wistar rats were randomly divided to three groups: control group received 2ml of distilled water and first and second treatment groups received 30 and 45 mg/kg buprenorphine every day intraperitoneally for 21 days. The rats were killed humanly at the end of treatment period and their testes were removed and weighed. For histopathological evaluations, tissue sections stained by Hematoxylin and Eosin (H&E). The result obtained for the 3 groups were compared using nonparametric Kruskal-Wallis test. P<0.05 was considered the level of significance.

Results: The volume of testis in the treatment group 2 in comparison with control and treatment group 1 showed a decrease and necrosis of seminiferous tubules and increasing of interstitial tubules in treatment group 2 in comparison with the other two groups showed a significant increase. Number of seminiferous tubules, leydig-cells, thickness of germinal epithelium, spermiogenesis index, tubules differentiated index in treatment groups 1 and 2 was significantly lower than the control group (p<0.05).

‍Conclusion: Using buprenorphine drug can increase infertility to reasons, decrease spermatogenesis and necrosis on seminiferous tubules.