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The study of attachment factor and biofilm formation of uropathogenic Escherichia coli isolated from patient with urinary tract infection of Semnan Province
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Nazila Arbab Soleimani   , Zahra Amini * , Elahe Tajbakhsh |
| Department of Microbiology, Damghan Branch, Islamic Azad University, Damghan, Iran , Zahra_amini_63@yahoo.com |
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Abstract: (9739 Views) |
| Background and Aim: Urinary tract infection (UTI) is the most common nosocomial infection, which is caused by colonization of uropathogenic Escherichia coli to host mucosal epithelium and damage host tissue. Materials and Methods: In this study 100 samples were collected from patients who suspected urinary tract infection. Antibiotic resistance of uropathogenic Escherichia coli was done by Kirby-Bauer method. Biofilm formation of this bacterium was performed by micro-titer plate method. To study the attachment of bacteria, cell surface hydrophobicity of uropathogenic Escherichia coli was done by hydrocarbon octane method. Results: Among 100 examined samples using conventional biochemical methods, 70 samples were identified as uropathogenic Escherichia coli. Results showed that, this bacteria with 23% hydrophobicity was able to form biofilm. Among these, 60% showed strong potential to form biofilm and just 4% did not have such potential. |
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| Keywords: Urinary tract infections, Uropathogenic Escherichia coli, Biofilm formation |
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Full-Text [PDF 302 kb]
(4420 Downloads)
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Type of Study: Original |
Subject:
Medicine
Received: 2017 | Accepted: 2017 | Published: 2017
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