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Determination of genotype variation of Escherichia coli in well water of Tehran’s parks by Multiplex PCR
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Mohammad Mehdi Soltan Dallal *   , Saman Sepehri , Saman Sepehri , Akram Tabatabaei Bafrouei5 , Zahra Deilami Khiabani |
| Professor, Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran , soltanirad34@yahoo.com |
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Abstract: (8830 Views) |
| Background and Aim: Water plays a major role in public health and the contaminated water has direct effect on human health. E.coli is an indicator of water pollution different strains of E.coli such as ETEC, EHEC, EIEC and EPEC can cause diarrhea in children. The aim of this study is to investigate the contamination rate of well water of Tehran’s parks, and pathotyping E.coli strains which cause diarrhea. Materials and Methods: 156 well water samples from five different geographical parts of Tehran (north, south, east, west, and the centre) were collected aseptically. The collected samples were transferred to the laboratory in microbiology section, School of Public Health, for membrane filtrations and patotyping. Results: 90 out of 156 samples (54/5%) were contaminated with E.coli. The samples from south part were more contaminated compared to others. Out of 90 isolated E.coli only 67 were DEC, in which 42 (62.7%) were detected as EPEC, 12 (17.9%) as STEC or ETEC, 9 (13.4%) as EIEC and 4 (6%) as ETEC by PCR method. Conclusion: Presence of different biotypes of E.coli in well water of parks can be dangerous, especially for children thus, placing warning signs and educating people in this regard should be taken seriously. |
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| Keywords: Water Wells, Escherichia coli, Genetic Testing, Diarrhea |
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Full-Text [PDF 244 kb]
(3917 Downloads)
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Type of Study: Original |
Subject:
Medicine
Received: 2017 | Accepted: 2017 | Published: 2017
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