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Surveying the Frequency of β-lactamase Enzyme and Antibiotic Sensitivity Pattern in Isolated Pathogen Bacteria from Low and High Hospital Contact Surfaces
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Sheila Jalalpour *   , Rooha Kasra Kermanshahi , Ashraf Sadat Nouhi , Hamid Zarkesh Esfahani |
| , shilla.jalalpoor@yahoo.com |
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Abstract: (11602 Views) |
| Background and Aim: Hospital surfaces can serve as reservoirs of potential pathogen bacteria. β-lactam antibiotics are developed as wide-spectrum antibiotics, however, the emergence of bacteria encompassing β-lactamase was a major challenge for physicians. The present study was conducted to determine the frequency of β-lactamase and antibiotic sensitivity pattern in isolated pathogen bacteria from low and high hospital contact surfaces. Material and Methods: This experimental study was conducted on 194 isolated samples in Azzahra hospital in Isfahan. Samples were collected from high and low contact surface with swab in NB (Nutrient Broth). Bacteria were identified with microbiological techniques, however, β–lactamase production was assessed with acidometric and antibiogram pattern was achieved with Kirby Bauer method. Results: Of 194 isolated bacteria, the following organisms were detected: Staphylococcus spp. 55%, Bacillus spp. 26.3%, Enterobacteriaceae 9.8%, Pseudomonas spp. 3.9%, other gram negative bacteria 4.5% and Streptococcus spp. 0.5%. Meanwhile, 61.5% of bacteria produce β–lactamase. According to antibiogram, organisms showed the highest and lowest resistance to penicillin and gentamicin, respectively. Conclusion: Widely spread of β–lactamase represents high spread of bacteria in hospital surfaces. Obviously, improvement in the quality of disinfectant agents could pave the way for better management of antibiotic-resistant nosocomial infections. |
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| Keywords: β-lactamase, Antibiotic resistance, Hospital surfaces, Nosocomial infections |
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Full-Text [PDF 200 kb]
(1835 Downloads)
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Type of Study: Original |
Subject:
Medicine
Received: 2017 | Accepted: 2017 | Published: 2017
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