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Comparsion between Cation-Exchange Chromatography Using HbGold IVD and Reference Method of Micro-Chromatography
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Mohammadi Torbati P *   , Rastegar Rad N |
| , p2000torbati@yahoo.com |
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Abstract: (11432 Views) |
| Background: Accurate and precise hemoglobin separation and quantitation of HbA2 and HbF are essential for diagnosis of thalassemia. To evaluate the potential application of cation-exchange chromatography by HbGold IVD in performing hemoglobin A2 quantificative assay, comparsion between this technique and reference method of micro-chromatography was carried out. Materials and Methods: The blood samples were obtained from individuals who had either thalassemia trait or healthy status (n=321). Results: Variability of HbA2 quantitation was quite low the CVs of within-run between run & interlaboratory studies were:1.8-3.1% , 3.4-6% and 6.6-8.8%, respectively. The results of HbA2% quantitated by HbGold analyzer correlated with those given by Helena microchromatogrophy system (r=0.974.p=0.001). Conclusion: The HbGold was simple, rapid and reproducible. The application of HbGold analyzer for disgnosis of various phenotypes of thalassemia (the ailment which is frequently observed in Iran) is considered. In conclusion the HbA2 assay of HbGold analyzer could be used for both the quantition of HbA2 and the presumptive identification of thalassemia. |
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| Keywords: HbA2, Cation Exchange Chromatography, Microchromatography, HbGold |
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Full-Text [PDF 127 kb]
(2279 Downloads)
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Type of Study: Original |
Subject:
Medicine
Received: 2017 | Accepted: 2017 | Published: 2017
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