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The occurrence of TEM gene among the extended-spectrum β-lactamases (ESBLs) producing uropathogenic Escherichia coli strains, isolated from Khorramabad city in 2013
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Gholamreza Goudarzi *   , Azin Baharvand Ahmadi1 |
| , Goudarzi.gh@gmail.com |
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Abstract: (6191 Views) |
| Abstract: Background and Aim: The extended-spectrum β-lactamases (ESBLs) are mutant enzyme variants, which primarily derived from broad-spectrum β-lactamases TEM 1, 2 and SHV1. They have a wide substrate profile, allowing hydrolysis of all cephalosporins, penicillins and aztreonam. There is remarkable geographical diversity in the occurrence of ESBLs in Iran. The current study was investigated to look for the presence of TEM gene among ESBLs-positive uropathogenic Escherichia coli (UPEC) isolates in Khorramabad city, Iran in 2013. Materials and Methods: In this cross-sectional study, a total of two–hundred uropathogenic E. coli isolates were collected and identified from selected hospitals of Khorramabad city. Screening and confirmation of ESBL production was phenotypically carried out according to the Clinical and Laboratory Standards Institute (CLSI) recommended methods. The frequency of TEM gene among ESBLs-positive isolates was determined using PCR technique. Results: Out of 200 uropathogenic E. coli, 91 (45.5%) isolates were shown to produce ESBLs as investigated by disk diffusion test, which among them, 60 (65.9%) isolates carried TEM genes as detected by PCR. Conclusion: The frequency of ESBL-positive UPEC isolates, as well as harboring TEM is high in Khorramabad city. This subject is a serious threat. Therefore, it may be resulted in the treatment failure. In addition, at least in our region, carbapenems (e.g. imipenem) can be as an antibiotic choice to eradicate ESBLs-producing UPEC isolates. We recommend the hospitals to perform laboratory methods for detection ESBLs-positive isolates, as well as antibiotic prescription policy, particularly in cases of empirical therapy to be revised. |
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| Keywords: PCR, Escherichia coli, TEM, Extended-spectrum β-lactamases |
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Full-Text [PDF 225 kb]
(1316 Downloads)
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Type of Study: Original |
Subject:
Medicine
Received: 2017 | Accepted: 2017 | Published: 2017
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