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Molecular detection of Chlamydia psittaci in fecal samples of asymptomatic parrots in Mazandaran, Iran
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Mohammad Reza Mahzounieh   , Heidar Heidari Khoei * , Mahsa Niknejad , Fatemeh Yektaneh |
| . DVM Student, Faculty of Veterinary Medicine and Research Institute of Zoonotic Diseases, Shahrekord University, Shahrekord, Iran , heidarheidari@yahoo.com |
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Abstract: (7981 Views) |
| Background and Aim: Psittacosis (Chlamydiosis), is a zoonotic disease in birds caused by Chlamydia psittaci, an obligatory intracellular bacterium and member of the family Chlamydiaceae. The clinical signs depend on the species and age of the avian as well as the pathogenicity of strain of C. psittaci. The infections can manifest as an acute, or chronic illness, but can also be asymptomatic. C. psittaci can persist in the host for long periods, often without causing obvious illness, and therefore poses a threat for public health. Thus, an outbreak may happen through direct contact or inhalation of infected aerosols and dried faces. The aim of this survey was molecular detection of C. psittaci in fecal samples from different species of parrots in Iran. Materials and Methods: A total of 32 fecal samples were collected from different species of parrots in Iran. DNA was extracted from fecal samples using DNPTM kit (CinnaClon Co., Iran) according to manufacturer’s instructions. Two parts of C. psittaci DNA was detected using two sets of primers, which target a 432 bp, and 127 bp fragments of bacterial DNA in the first- and second-rounds of PCR, respectively. Results: After PCR amplification of extracted DNA from samples, 8 (25%) samples out of 32 fecal samples were found positive for presence of C. psittaci DNA. Conclusion: These results indicate the medium prevalence of sub-clinical psittacosis in Iranian psittacine populations, which consequently could pose a zoonotic hazard to public health. Since many people are in close contact with these birds more public education about the disease is necessary. More studies should be done to find more information like predominant genotypes of C. psittaci in the parrot population of Iran. |
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| Keywords: Chlamydia psittaci, Psittacine birds, Nested-PCR, Zoonoses, Iran |
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Full-Text [PDF 310 kb]
(2478 Downloads)
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Type of Study: Original |
Subject:
Medicine
Received: 2017 | Accepted: 2017 | Published: 2017
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