|
Antimicrobial effect of plant peptide MBP-1 and silver nanoparticles, along with their synergistic effect on skin infection due to Pseudomonas aeruginosa: in vitro and animal model
|
Fatemeh Mirzaei   , Mojtaba Salouti * , Reza Shapouri , Zahra Heidari |
| . Associate Professor, Biology Research Center, Islamic Azad University, Zanjan Branch, Zanjan, Iran , saloutim@yahoo.com |
|
|
Abstract: (8162 Views) |
| Background and Aim: Pseudomonas aeruginosa is an opportunistic pathogen with innate resistance to many antibiotics and disinfectants that cause severe, acute and chronic nosocomial infections in immunocompromised, catheterized or burn patients. MBP-1 is a plant antimicrobial peptide that has showed antimicrobial activity against some bacteria and filamentous fungi. The antimicrobial effect of silver nanoparticles against a range of microorganisms have also been reported. The aim of the present study was to investigate the synergistic effect of MBP-1 and silver nanoparticles combination against skin infection caused by Pseudomonas aeruginosa. Materials and Methods: The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of MBP_1, silver nanoparticles and their combination was measured based on the microdilution and macrodilution susceptibility test. The synergistic effect of MBP-1 and silver nanoparticles combination was investigated using 12 mice with induced bacterial skin infection using Pseudomonas aeruginosa. Results: The results showed that MIC and MBC of silver nanoparticles for P.aeruginosa were 3.12 and 6.25 ppm, respectively. The MIC and MBC of plant peptide MBP-1 were 500 and 600 µg/ml, respectively. MIC and MBC of MBP-1 and silver nanoparticles combination on P.aeruginosa were 400 µg/ml, 1.56 ppm and 500 µg/ml, 3.125 ppm, respectively. Therefore, antimicrobial effect of MBP-1, silver nanoparticles and their combination against skin infections due to P.aeruginosa was approved in mouse model. Conclusion: The results showed that MBP_1 and silver nanoparticles have antimicrobial effect against P.aeruginosa and their combination has synergistic effect to heal skin infection caused by P.aeruginosa in mouse model. |
|
| Keywords: Pseudomonas aeruginosa, Skin Diseases, Infectious, MBP-1 protein, Zea mays, Silver Compounds, Nanoparticles, Synergistic Effect |
|
|
Full-Text [PDF 419 kb]
(2464 Downloads)
|
Type of Study: Original |
Subject:
Medicine
Received: 2017 | Accepted: 2017 | Published: 2017
|
|
|
|
|
|
