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Does inhalation of formaldehyde lead to oxidative stress in blood of rat?
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Mohammad Ranjbarian   , Ali Khavanin , Asghar Dehghani * , Mansour Rezazadeh Azari , Abdolamir Allameh , Siamak Sabour , Faezeh Abbas Gohari , Sara Azizian |
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Abstract: (8222 Views) |
| Background: Formaldehyde (FA) is a highly-used chemical in industries and hospitals. The aim of this study was to explore the status of oxidant and antioxidant parameters in serum of rats exposed to FA inhalation. Materials and methods: Twenty-one male Wistar albino rats divided into 3 separate groups randomly (n=7). Rats in groups 1 and 2 were respectively exposed to atmospheres containing 6 and 12 PPM FA continuously (8 hours/day, 14 days), while animals in group 3 which served as control group were not exposed to any stress source. The animals were anesthetized by CO2 and after decapitation, blood samples were collected and processed for estimation of biochemical parameters in control and exposed groups. The level of MDA and GSH in serum was measured respectively by TBA reagent and Beutler's method by spectrophotometric method. Results: The present research findings showed statistically significant body weight loss in FA (12ppm) exposed group compared to the control and also FA (6ppm) exposed groups. However, a statistically significant decrease in liver/body weight ratio was observed in the exposed groups compared to the control group (p<0.05). FA exposed groups showed a statistically significant decrease in GSH level in serum and a statistically significant increase in MDA level in serum compared to control group (p<0.05). However, the levels of changes in biochemical parameters in FA exposed groups were not dose-dependent. Conclusion: The results of present study show that exposure to FA is toxic to blood. Further research for exploring the toxicity of FA in occupationally exposed groups is recommended. |
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| Keywords: Formaldehyde, Oxidative stress, Reduced Glutathione, Lipid peroxidation, Malondialdehyde |
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Full-Text [PDF 556 kb]
(3082 Downloads)
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Type of Study: Original |
Subject:
Medicine
Received: 2017 | Accepted: 2017 | Published: 2017
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